乙醇适应对鼠伤寒沙门氏菌及其菌膜耐致死胁迫的影响

发布时间：2019/11/4 15:55:32     浏览次数：1084

乙醇适应对鼠伤寒沙门氏菌及其菌膜耐致死胁迫的影响
Effects of Ethanol Adaptation on the Resistance to Lethal Stress of Salmonella typhimurium and Its Biofilm
  
DOI：
中文关键词: 鼠伤寒沙门氏菌  菌膜  乙醇适应  苹果酸

英文关键词: Salmoenlla typhimurium  biofilm  ethanol adaptation  malic acid

本文主要研究鼠伤寒沙门氏菌在玻璃表面生物菌膜（BF）的生长特性和乙醇作用方式对鼠伤寒沙门氏菌菌膜形成的影响以及乙醇适应处理对浮游菌与菌膜耐致死胁迫的作用。采用结晶紫染色观察鼠伤寒沙门氏菌菌膜的形成，用酶标仪测量波长570 nm处不同条件下生物菌膜的生物量，用平板菌落计数衡量乙醇适应处理对浮游菌及菌膜耐致死胁迫的影响。结果表明，结晶紫染色可清晰观察到鼠伤寒沙门氏菌在玻璃表面形成的生物菌膜，菌膜形成的紧密网状结构随着时间的延长而增强。乙醇作用方式对菌膜形成有显著影响，加入不同含量的乙醇后培养48 h，乙醇对菌膜形成有显著的抑制作用。预先培养菌24 h后加入5%的乙醇，能显著促进菌膜的生长。菌膜形成过程中，当营养条件为1/10 TSB，5%乙醇适应能增加菌体对苹果酸的耐受性，增加浮游菌对12%的乙醇及5 mg/mL苹果酸的耐受性。

  The objectives of this study were to investigate the growth characteristics of Salmonella typhimurium biofilm（BF） on glass surface， effect of ethanol activity on the formation of S. typhimurium BF and effect of ethanol adaptation on the resistance of planktonic and BF to lethal stress. The morphology of S. typhimurium BF on the glass surface was observed by crystal violet staining， BF biomass by Thermo Scientific Microplate Reader at 570 nm and ethanol adaptation by plate colony count. Results showed that S. typhimurium BF formed on the glass surface could be directly observed by crystal violet staining， and reticular structure formed by S. typhimurium BF became denser with incubation time. Ethanol activity had significantly different effects on the formation of BF. Ethanol added at different level during 48 h incubation of S. typhimurium inhibited the formation of BF significantly， however， ethanol added after the incubation of S. typhimurium for 24 h at 5% level only promoted the growth of BF. Ethanol at level of 5% increased the tolerance of bacteria to malic acid in BF formation under the condition of 1/10 TSB. In addition， 5% ethanol adaptation increased planktonic resistance to 12% ethanol and 5 mg/mL malic acid.