大豆异黄酮纳米粉体的反溶剂法制备及表征

发布时间：2019/11/4 15:57:25     浏览次数：1161

大豆异黄酮纳米粉体的反溶剂法制备及表征
Preparation and Characterization of Soy Isoflavone Nanoparticles by Antisolvent Precipitation Method
  
DOI：
中文关键词: 大豆异黄酮  微细粉体  反溶剂  表征  粒径

英文关键词: soy isoflavone  powder  anti-solvent  characterization  particle size

以二甲基亚砜作为溶剂，丙酮为反溶剂制备大豆异黄酮纳米粉体。通过正交试验设计法，以大豆异黄酮粒径为指标，对各个影响因素和水平进行研究，得到最佳的试验条件。结果：大豆异黄酮的纳米粒径与溶液体积比、匀浆器转速、溶液浓度呈反比，与反应时间呈正比。大豆异黄酮的最佳试验条件为：溶液质量浓度300 mg/mL，转速1 200 r/min，溶剂与反溶剂的体积比1∶6，反应时间3 min。在该条件下得到的纳米大豆异黄酮粒径为98 nm。通过扫描电镜（SEM）观测粒子外观，并用粉体堆密度方法统计。通过红外光谱（FT-IR）、差热扫描（DSC）等方法对制备的粉体进行物理化学性质表征。结果表明：制备前、后大豆异黄酮的粉体化学性质没有发生改变，纳米大豆异黄酮颗粒分布均匀，粒径显著减小。

Taking dimethyl sulfoxide as solvent， soy isoflavone powder was prepared by acetone as the anti-solvent. Orthogonal experimental design method was used to in spect the major factors affecting the size and morphology of soy isoflavone powder. Results show that the particle size has a negative relationship with volume ratio， rotor speed and concentration， and has a positive relationship with reaction time. Experiment indicates that the measured powder particle size reaches 98 nm when the concentration is 300 mg/mL； rotor speed is 1 200 r/min； volume ratio is 1 ∶ 6 and reaction time is 3 min. Isoflavone and nano-isoflavone power were evaluated by using scanning electron microscope （SEM）， infrared spectroscopy （FT-IR）， differential scanning calorimetry （DSC） and statistics of powder heap density. Results showed that the chemical properties of isoflavones did not change after preparation. Nano-isoflavone particles were distributed evenly and the particle size decreased significantly.